论文代写:神经细胞培养

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18/03/2018

论文代写:神经细胞培养

培养的神经细胞是产生神经元的体外培养方法,有助于促进对神经系统的理解。神经元细胞的培养具有挑战性,因为成熟的神经元不会进行细胞分裂。克服这个问题的方法是通过建立来自神经元肿瘤的辅助系。这些具有能够在细胞培养物中生长的优点。另外,它们具有无限的细胞数量,以最大限度地减少不同文化之间的差异。其局限性在于细胞类型的许多重要的生理差异以及在何处形成的基础。这些诱导的细胞系显示神经元表型。这是由细胞时间内的重要生理差异完成的。细胞系以它们通过操纵培养条件显示神经元表型的方式被诱导。这些是通过添加某些生长因子实现的。考虑使用所讨论的SH-SY5Y成神经细胞瘤细胞是必要的。修改后的协议用于提供原始培养条件。现在使用这些用于SH-SY5Y细胞的正常培养条件,并发现它们在很大程度上没有改变。观察到SH-SY5Y细胞以不会未分化的细胞连续生长。

论文代写:神经细胞培养
它们具有与神经母细胞形态相似的结构并且表达未成熟的神经元标记。在这个模式中,很明显,已建立的神经元市场将不会被使用。与神经细胞形态共振的类似结构与原代神经元相似。这些是较长过程的一部分,并且它们表现出增殖速率的降低。他们退出细胞周期和性别G0。很明显,在这种情况下观察到某些神经元特异性标记的表达。许多其他辅助细胞系用于研究神经元细胞。观察神经元细胞系并认为其可用于研究神经元细胞系和培养物。他们在当前使用。优选使用原代培养物,因为它们不是来源于肿瘤细胞。他们更有可能在体内发展神经元细胞的特性。原代细胞系培养难以发展。动物组织由几种不同种类的细胞组成。除此之外,大脑并不是神经元所处文化发展的唯一来源。复杂的神经元网络结合在胃肠道内的肠道控制壁内。这些形成肠神经系统。这些也被称为第二脑。在神经元的隔离中呈现肠神经元的分离。培养神经元细胞的一个重要问题是成熟的神经元不会沉迷于增殖。

论文代写:神经细胞培养

Cultured neurosn is the methods of producing the neurons in vitro cultures are instrumental to the advancing of the understanding of the nervous system. The culture of the neuronal cells is challenging since mature neurons does not do cell division. The ways to overcome this issue are by establishing of the secondary lines that are derived from the neuronal tumours. These have the advantage of being able to grow in the cell cultures. In addition, they have unlimited cell numbers in the minimizing of the variability between the different cultures. The limitation is that many important physiological differences with the cell types and the basis of where are formed. These cell lines which are induced display neuronal phenotypes. This was done by the important physiological differences within the cell times from which they were derived. The cell lines are induced in a manner where they would display neuronal phenotypes by the manipulation of the culture conditions. These were achieved by the addition of certain growth factors. It is imperative to consider the use of the SH-SY5Y neuroblastoma cells that are discussed. Modified protocol is used with the presenting of the original culturing conditions. These normal culture conditions that are used for the SH-SY5Y cells are now used and are found to be largely unchanged. SH-SY5Y cells are observed grow continuously as cells that will not be undifferentiated.

论文代写:神经细胞培养
They have a structure that is similar to the neuroblast morphology and are to express immature neuronal markers. In this schema, it is evident that the established neuronal markets will not be used. The similar structures that resonate with the neuroblast morphology are similar to the primary neurons. These were a part of a longer process and they exhibit a reduction in the proliferation rate. They exit cell cycle and gender the G0. It is evident that expression of certain neuron-specific markers that are viewed in this condition. Numerous other secondary cell lines are used for the study of the neuronal cells. The neuronal cell lines are observed and considered to be useful in the study of the neuronal cell lines and cultures. They are used in the current times. The use of the primary cultures is preferred as they are not derived from the tumour cells. They are more likely to develop the properties of the neuronal cells in vivo. Primary cell line cultures are difficult and challenging to develop. The animal tissues are made of several different kinds of cells. Added to this, the brain is not the only source for development from which the neurons are cultures. Complex networks of neurons are combined within the walls of the gut control within the gastro intestinal tract. These form the enteric nervous systems. These are also dubbed as second brain. Isolation of the enteric neurons is presented in the isolation of the neurons of the brain. One important issue in the culturing of the neuronal cells is that the mature neurons do not indulge in proliferation.

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