对于第二个问题，HO面积大小将直接或间接受到V1大小的影响。高阶区域与相关的初级感觉区域如初级视觉区域V1呈线性关系(Chou et al .， 2013;Harris和mr sici – flogel, 2013)。其次，以V1大小的双向变化为模型，研究表明，皮层属性等高阶功能的实现不是由于体积的增大，而是由于复杂度的增加。
在这种情况下，假设有一个基因只在初级视觉皮层表达。在删除后会导致初级视觉皮层V1变小的情况下，可以使用作者假设进行类似的实验。取胚胎发育阶段带有geneX基因的突变小鼠，研究其主要视觉区域，并与正常小鼠进行比较(Harris, and mrsici – flogel, 2013;(Arnal, and Giraud, 2012)， geneX的作用将展示遗传机制如何影响V1的生长。其次，对正常水平的geneX小鼠进行研究，然后对剔除geneX的小鼠进行研究。根据作者的论点，即使这种形式的实验表明V1的大小在没有geneX的地方减小，视觉皮层的功能也不会改变。
Size of other sensory and motor areas will change in a linear way. RNA in-situ hybridization was used for quantification purposes (Kok et al, 2012; Liang et al, 2013). Two likely results that can be identified are that of the linear relationship which is identified between size of primary visual area and higher order visual areas as triggered by genetic mechanisms. And secondly, higher order areas will scale linearly with the primary sensory areas.
For the second question, HO area size would be directly or indirectly influenced by the size of V1. Higher order areas would scale linearly with related primary sensory areas such as primary visual areas V1 (Chou et al, 2013; Harris, and Mrsic-Flogel, 2013). Secondly, with bi-directional changes of V1 size as a model, the study reveals that higher order functions such as cortex properties are not achieved because of increase in size but in complexity.
Now in this context, assuming that there is geneX which is expressed exclusively in the primary visual cortex. On the condition that if deleted, it could lead to the decrease in size of the primary visual cortex V1, then a similar experiment could be conducted using author hypothesis. Where mutant mice with geneX in embryonic development would be taken and their primary visual area would be studied and compared against that of normal mouse (Harris, and Mrsic-Flogel, 2013; Arnal, and Giraud, 2012), the effect of geneX would show how the genetic mechanism would have an effect on the growth of V1. Secondly, mouse with normal levels of geneX would be studied, and then thirdly, mouse with the geneX removed would be studied. Keeping to the arguments made by the authors, even if this form of an experiment showed that size of V1 decreases where there is not geneX, the functionality of the visual cortex would not change.